Interaction between Highly Diluted Samples, Protein Solutions and Water in a Controlled Magnetic Field

" We have previously shown that water incubated in a weak combined magnetic field (CMF) increased the production of reactive oxygen species (ROS) by neutrophils. Adding high dilutions (HD) of water into the same system resulted in a similar effect. HD of antibodies to interferon-gamma (HD Abs to IFNγ) were shown to emit electromagnetic radiation and affect hydrogen bond energies. Here, we aimed to evaluate the effect of HD of substances (donor) on the properties of aqueous solutions (acceptor). The donor and acceptor were incubated for 1 h in a controlled magnetic field so that the walls of the two cuvettes were in close contact. As a control, the acceptor was incubated under the same conditions but without the donor... Joint incubation led to a 185–356% increase (p < 0.05) in ROS production, depending on the type of acceptor sample. The magnitude of the effect depended on the parameters of the magnetic field. In a CMF, the effect was strongest, completely disappearing in a magnetic vacuum or with shielding. These findings are important for understanding the physical mechanism of action of HD preparations, which opens up opportunities for expanding their practical applications." {Credits 1}

" The physical (quantum) nature of the distant interaction of samples is clearly shown in Figure 5, where placing donor and acceptor samples side by side (columns 3 and 4) leads to an approximately twofold increase in the chemiluminescence intensity compared to similar samples separated from each other (columns 1 and 2). Moreover, it should be noted that there is not much difference in the magnitude of the effect between the pair of the samples either completely wrapped (columns 5 and 6) or not wrapped in aluminum foil (columns 3 and 4). In the absence of foil (columns 3 and 4, Figure 5), the effect is much more pronounced (equally for the donor and acceptor), and when the cuvettes are wrapped in foil on all sides except the contact side of the cuvettes, the effect is much less pronounced and manifests itself differently for the donor and the acceptor (columns 7 and 8). To explain this effect, it can be assumed that aluminum foil shields the electrical component of electromagnetic radiation in the radio frequency range, in contrast to permalloy (the outer shield of the device used in the experiments), which is a magnetic shield mainly for SMF and low‐frequency AMF. In this regard, it can be assumed that the result obtained by us in experiments with shielding (changes in the distant effect when foil was partially wrapped around each cuvette) indicates the need for the presence of not only CMF with optimal parameters for implementing the distant effect, but also possibly the simultaneous presence of the electrical component of the electromagnetic field (EMF). Seemingly, in addition to the magnetic field, we discovered another factor that controls the distant interactions—EMF. This hypothesis also explains the differences in the relative values in pairs of samples 3 and 4, as well as 7 and 8 (Figure 5), where partial shielding with aluminum foil, in all likelihood, can to a certain extent shield the external electromagnetic effect on the samples. This is, no doubt, an important result that requires a special detailed study in the future." {Credits 1}

" Thus, the results obtained in this work about the features of physical interactions between donor and acceptor samples are consistent with the results of a number of studies [3,4]. For example in [4], Preliminary cell exposure for several minutes to a microwave radiation field at 36 GHz inhibited a 47 Hz peak, and such a water state was retained after switching off the microwave field for dozens of minutes or hours, depending on the radiation power [4]. Another study demon‐strated that the aqueous solution remembers the effect of microwave radiation (42 GHz). In these experiments the effect manifested itself in a change in the opening frequency of Ca(2+)‐dependent K+ channels in the presence of water after radio exposure [3]. Thus, it has been shown that not only the electromagnetic but also the magnetic component has its own biophysical effect." {Credits 1}

" In our studies [23–25], reproduced by other laboratories [26–29], it was shown that the effect of a weak CMF on aqueous solutions of amino acids (ionic current reaction) directly depends on the presence and direction of the electric field (in this case, set by the inter‐electrode potential difference). This potential difference determines the energy transfer and its direction in the physical and chemical system under study. By analogy with the results of these experiments, it can be assumed that radio frequency EMF, in the case of the distant effect discussed here, is necessary for a more efficient polarization of the medium, and this, in turn, is a necessary condition for a finer control action of CMF. All this together creates necessary background for distant interaction (exchange of photons) between HD samples, protein solutions, and water." {Credits 1}

" It has already been found that EMF in the single‐digit GHz range polarizes protein macromolecules in an aqueous solution [30,31]. Interest in the combined action of magnetic fields and radio frequency EMF on biological objects increased in connection with the study of the biological action of magnetic fields, taking into account the hyperfine interaction between nuclei and electrons in the implementation of the mechanism of radical pairs (in this case, frequencies in the range of 1–15 MHz can be active) [32,33]. It should be noted that the number of indoor sources of radio frequency EMF, including in laboratories, has increased significantly over the past two decades, as mobile phones, Wi‐Fi, and other wireless technologies have become an integral part of our life. A large number of radio transmitters creates a constant multi‐spectral radio signal environment, exposing experimental objects to low‐power electromagnetic waves. In this situation, taking into account the data obtained by us, further studies will require a thorough control of the background electromagnetic environment and, possibly, the artificial formation of an optimal combination of electromagnetic parameters of different frequency ranges. Thus, in this paper we show that the use of an experimental device which allows the control and setting of the parameters of the magnetic field affects the results of the experiments (it increases the reproducibility and the magnitude of the effect). In conclusion, it should be noted that, not surprisingly, our screening study of the possibility of distant interactions between HD preparations, protein solutions, and water turned out to be very productive. The existence of a distant effect was demonstrated. We showed that the distant effect can depend on accompanying processes, such as the influence of absorbing atmospheric gases into pure water, as well as on the interaction of magnetic and electromagnetic factors that possibly control the ultra‐weak photon emission by solutions and, as a result, distant interaction. All the obtained data seem to us to be very promising for further in‐depth studies." {Credits 1}

{Credits 1} 🎪 Novikov, V.V.; Yablokova, E.V. Interaction between Highly Diluted Samples, Protein Solutions and Water in a Controlled Magnetic Field. Appl. Sci. 2022, 12, 5185. © 2022 the authors. This work is licensed under the Creative Commons Attribution International License (CC BY 4.0).