Wavelength- and irradiance-dependent changes in intracellular nitric oxide level

" In summary, these results imply that the various wavelengths of light potentially modulate NO levels by different mechanisms, and likely different chromophores. Specifically, although red and green individually resulted in the same overall percent increase in NO levels, one was not necessarily capable of substituting for the other. This implies that a full dose of red or green is required for a maximum effect, and that they operate by different mechanisms to drive NO release or production." {Credits 1}

" Even more striking, NIR and blue seem to complement each other when exposed simultaneously, and NIR exposure seems to potentiate the effect of a subsequent blue exposure. Thus, it would seem that to enhance a blue exposure, an NIR wavelength must be present at the beginning of the overall exposure duration. In contrast, blue does not have the same effect on a subsequent NIR exposure, which provides further evidence that different wavelengths are functioning by different mechanisms and chromophores." {Credits 1}

" It is often hypothesized that the immediate source of NO increases in PBM is release of NO from binding sites at COX, in turn allowing for downstream increases in ATP synthesis. However, given the large and long-lasting increases in NO observed (Fig. 2) and the relatively short half-life of NO in the cell,50 it is difficult to imagine that this is exclusively due to the release of already extant COX-bound NO." {Credits 1}

" Strikingly, with all wavelengths, the previously observed rise in NO levels seen at 90-min postexposure was completely absent in all cases when nNOS was inhibited. Additionally, NO levels decayed at a much faster rate than in cells without SKF-525A. This unequivocally demonstrates that the observed secondary increase and the long-term elevation of NO following light exposure are dependent on production of NO by nNOS in our cell system. Whether this NOS activity is due to traditionally spliced nNOS, mtNOS, or a combination of the two has yet to be determined." {Credits 1}

{Credits 1} 🎪 Nathaniel J. Pope, Samantha M. Powell, Jeffrey C. Wigle, Michael L. Denton, "Wavelength- and irradiance-dependent changes in intracellular nitric oxide level," J. Biomed. Opt. 25(8) 085001. © 2020 The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 Unported License.

Last modified on 18-Oct-20

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