Exposure of the SH-SY5Y Human Neuroblastoma Cells to 50-Hz Magnetic Field Comparison Between Two-Dimensional (2D) and Three-Dimensional (3D) In Vitro Cultures

" The culturing of primary neurons and neuronal-like cancer cells on two-dimensional (2D) surfaces represents the conventional in vitro experimental model for both oncologic and neurodegenerative disorders. The limitations of such growing conditions are recently emerging, as 2D cultures lack the complex anatomical and functional connectivity of the neuronal network that underlies both the physiological and pathological condition [1,2,3]. To provide a more functional, structural, and biochemical system that might closely resemble the in vivo environment, different three-dimensional (3D) matrices have been developed..." {Credits 1}

" In response to 72-h exposure to 50-Hz MF, we demonstrated that no proliferation change and apoptosis activation occur in both 2D and 3D cultures. Consistently, no modulation of Ki67, MYCN, CCDN1, and Nestin, of invasiveness and neo-angiogenesis-controlling genes (HIF-1α, VEGF, and PDGF) and of microRNA epigenetic signature (miR-21-5p, miR-222-3p and miR-133b) is driven by ELF exposure. Conversely, intracellular glutathione content and SOD1 expression are exclusively impaired in 3D-culture cells in response to the MF, whereas no change of such redox modulators is observed in SH-SY5Y cells if grown on 2D monolayers. Moreover, ELF-MF synergizes with the differentiating agents to stimulate neuroblastoma differentiation into a dopaminergic (DA) phenotype in the 3D-scaffold culture only, as growth arrest and induction of p21, TH, DAT, and GAP43 are reported in ELF-exposed SH-SY5Y cells exclusively if grown on 3D scaffolds." {Credits 1}

{Credits 1} 🎪 Consales, C., Butera, A., Merla, C. et al. Exposure of the SH-SY5Y Human Neuroblastoma Cells to 50-Hz Magnetic Field: Comparison Between Two-Dimensional (2D) and Three-Dimensional (3D) In Vitro Cultures. Mol Neurobiol (2020). © 2020 The Author(s). This article is licensed under the Creative Commons Attribution License.