Experimental Study on Blue Light Interaction with Human Keloid-Derived Fibroblasts

" Keloids are an exuberant response to wound healing, characterized by an exaggerated synthesis of collagen, probably due to the increase of fibroblasts activity and to the reduction of their apoptosis rate: currently no standard treatments or pharmacological therapies are able to prevent keloid recurrence." {Credits 1}

" Micro-Raman spectroscopy was used to explore direct effects of the blue LED light on the Cytochrome C (Cyt C) oxidase. We also investigated the effects of the irradiation on ionic membrane currents by patch-clamp recordings. Our results showed that the blue LED light can modulate cell metabolism and proliferation, with a dose-dependent behavior and that these effects persist at least till 48 h after treatment. Furthermore, we demonstrated that the highest fluence value can reduce cell viability 24 h after irradiation in keloid-derived fibroblasts, while the same effect is observed 48 h after treatment in perilesional fibroblasts. Electrophysiological recordings showed that the medium dose (20.6 J/cm2) of blue LED light induces an enhancement of voltage-dependent outward currents elicited by a depolarizing ramp protocol." {Credits 1}

{Credits 1} 🎪 Magni, G.; Banchelli, M.; Cherchi, F.; Coppi, E.; Fraccalvieri, M.; Rossi, M.; Tatini, F.; Pugliese, A.M.; Rossi Degl’Innocenti, D.; Alfieri, D.; Matteini, P.; Pini, R.; Pavone, F.S.; Rossi, F. Experimental Study on Blue Light Interaction with Human Keloid-Derived Fibroblasts. Biomedicines 2020, 8, 573. © 2020 The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 License.